Genetic variability of hepatitis C virus before and after combined therapy of interferon plus ribavirin
NAGIOS: RODERIC FUNCIONANDO

Genetic variability of hepatitis C virus before and after combined therapy of interferon plus ribavirin

DSpace Repository

Genetic variability of hepatitis C virus before and after combined therapy of interferon plus ribavirin

Show simple item record

dc.contributor.author Cuevas Torrijos, José Manuel
dc.contributor.author Torres Puente, Manoli
dc.contributor.author Jiménez Hernández, Núria
dc.contributor.author Bracho Lapiedra, Maria Alma
dc.contributor.author García Robles, Inmaculada
dc.contributor.author Wróbel, Borys
dc.contributor.author Carnicer, Fernando
dc.contributor.author Olmo, Juan del
dc.contributor.author Ortega González, Enrique
dc.contributor.author Moya, Andrés
dc.contributor.author González Candelas, Fernando
dc.date.accessioned 2010-07-13T08:01:03Z
dc.date.available 2010-07-13T08:01:03Z
dc.date.issued 2008
dc.identifier.uri http://hdl.handle.net/10550/15986
dc.description.abstract We present an analysis of the selective forces acting on two hepatitis C virus genome regions previously postulated to be involved in the viral response to combined antiviral therapy. One includes the three hypervariable regions in the envelope E2 glycoprotein, and the other encompasses the PKR binding domain and the V3 domain in the NS5A region. We used a cohort of 22 non-responder patients to combined therapy (interferon alpha-2a plus ribavirin) for which samples were obtained before initiation of therapy and after 6 or/and 12 months of treatment. A range of 25-100 clones per patient, genome region and time sample were sequenced. These were used to detect general patterns of adaptation, to identify particular adaptation mechanisms and to analyze the patterns of evolutionary change in both genome regions. These analyses failed to detect a common adaptive mechanism for the lack of response to antiviral treatment in these patients. On the contrary, a wide range of situations were observed, from patients showing no positively selected sites to others with many, and with completely different topologies in the reconstructed phylogenetic trees. Altogether, these results suggest that viral strategies to evade selection pressure from the immune system and antiviral therapies do not result from a single mechanism and they are likely based on a range of different alternatives, in which several different changes, or their combination, along the HCV genome confer viruses the ability to overcome strong selective pressures. en
dc.language.iso en en
dc.relation http://ukpmc.ac.uk/ptpmcrender.cgi?aid=1635067&blobtype=pdf en
dc.source Cuevas, JM; Torres-Puente, M; Jiménez-Hernández, N; Bracho, MA; García-Robles, I; Wrobel, B; Carnicer, F; del Olmo, J; Ortega, E; Moya, A; González-Candelas, F. Genetic variability of hepatitis C virus before and after combined therapy of interferon plus ribavirin. En PLoS One. 2008 Aug 26;3(8):e3058. en
dc.subject Hepatitis C virus; Genetic variability; Interferon; Ribavirin; Combined therapy en
dc.title Genetic variability of hepatitis C virus before and after combined therapy of interferon plus ribavirin en
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Genética ::Otras en
dc.identifier.doi 10.1371/journal.pone.0003058 en
dc.description.private cuevast@uv.es; mtorresp@uv.es; njimenez@uv.es; bracho@uv.es; garciai@uv.es; wrobel@uv.es; moya@uv.es; gonzalef@uv.es en

View       (388.9Kb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics