Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH
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Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH

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Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH

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dc.contributor.author Zaragozá Colom, Rosa
dc.contributor.author Miralles Fernández, Vicente
dc.contributor.author Rus, Diana
dc.contributor.author García de Mier, Concepción
dc.contributor.author Carmena Rodríguez, Rafael
dc.contributor.author Ruíz García-Trevijano, Elena
dc.contributor.author Barber Sanchis, Teresa
dc.contributor.author Pallardó, Federico V.
dc.contributor.author Torres Asensi, Luis
dc.contributor.author Viña Ribes, Juan
dc.date.accessioned 2010-06-29T10:34:34Z
dc.date.available 2010-06-29T10:34:34Z
dc.date.issued 2005
dc.identifier.uri http://hdl.handle.net/10550/13993
dc.description.abstract Zaragozá, R; Miralles, VJ; Rus, AD; García, C; Carmena, R; García-Trevijano, ER; Barber, T; Pallardó, FV; Torres, L; Viña, JR. At the end of lactation the mammary gland undergoes involution, a process characterized by apoptosis of secretory cells and tissue remodelling. To gain insight into this process, we analysed the gene expression profile by oligonucleotide microarrays during lactation and after forced weaning. Up-regulation of inflammatory mediators and acute-phase response genes during weaning was found. Expression of IκBα (inhibitory κBα), a protein known to modulate NF-κB (nuclear factor-κB) nuclear translocation, was significantly up-regulated. On the other hand, there was a time-dependent degradation of IκBα protein levels in response to weaning, suggesting a role for NF-κB. Furthermore, we have demonstrated, using chromatin immunoprecipitation assays, binding of NF-κB to the NOS-2 (inducible nitric oxide synthase) promoter at the early onset of events triggered during weaning. The three isoforms of NOS are constitutively present in the lactating mammary gland; however, while NOS-2 mRNA and protein levels and, consequently, NO production are increased during weaning, NOS-3 protein levels are diminished. Western blot analyses have demonstrated that protein nitration is increased in the mammary gland during weaning, but this is limited to a few specific tyrosine-nitrated proteins. Interestingly, inhibition of GSH synthesis at the peak of lactation partially mimics these findings, highlighting the role of NO production and GSH depletion during involution. en
dc.language.iso en en
dc.relation http://www.biochemj.org/bj/391/0581/3910581.pdf en
dc.source Zaragozá, R.; Miralles, J.V.; Rus, A.D.; García, C.; Carmena, R.; García-Trevijano, E.R.; Barber, T.; Pallardó, F.V.; Torres, L.; Viña, J.R. (2005) Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH. En Biochemical Journal. 391, 581–588 en
dc.subject GSH en
dc.subject involution en
dc.subject lactation en
dc.subject nitric oxide en
dc.subject NF-κB en
dc.title Weaning induces NOS-2 expression through NF-κB modulation in the lactating mammary gland: importance of GSH en
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Bioquímica en
dc.identifier.doi 10.1042/BJ20050507 en
dc.description.private zacoro@uv.es; mirallev@uv.es; cgmier@uv.es; carmena@uv.es, ; barber@uv.es; torresl@uv.es; vinaj@uv.es en
dc.identifier.idgrec 023286 en

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