A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal
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A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal

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A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal

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dc.contributor.author Gomis-Cebolla, Joaquín
dc.contributor.author Ricietto, Ana Paula Scaramal
dc.contributor.author Ferré Manzanero, Juan
dc.date.accessioned 2018-11-19T14:43:38Z
dc.date.available 2018-11-19T14:43:38Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10550/67954
dc.description.abstract The combined analysis of genomic and proteomic data allowed us to determine which cry and vip genes are present in a Bacillus thuringiensis (Bt) isolate and which ones are being expressed. Nine Bt isolates were selected from Spanish collections of Bt based on their vip1 and vip2 gene content. As a first step, nine isolates were analyzed by PCR to select those Bt isolates that contained genes with the lowest similarity to already described vip1 and vip2 genes (isolates E-SE10.2 and O-V84.2). Two selected isolates were subjected to a combined genomic and proteomic analysis. The results showed that the Bt isolate E-SE10.2 codifies for two new vegetative proteins, Vip2Ac-like_1 and Sip1Aa-like_1, that do not show expression differences at 24 h vs. 48 h and are expressed in a low amount. The Bt isolate O-V84.2 codifies for three new vegetative proteins, Vip4Aa-like_1, Vip4Aa-like_2, and Vip2Ac-like_2, that are marginally expressed. The Vip4Aa-like_1 protein was two-fold more abundant at 24 h vs. 48 h, while the Vip4Aa-like_2 was detected only at 24 h. For Vip2Ac-like_2, no differences in expression were found at 24 h vs. 48 h. Moreover, the parasporal crystal of the E-SE10.2 isolate contains a single type of crystal protein, Cry23Aa-like, while the parasporal crystal from O-V84.2 contains three kinds of crystal proteins: 7.0-9.8% weight of Cry45Aa-like proteins, 35-37% weight of Cry32-like proteins and 2.8-4.3% weight of Cry73-like protein.
dc.language.iso eng
dc.relation.ispartof Toxins, 2018, vol. 10, num. 5, p. 193-210
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Gomis-Cebolla, Joaquín Ricietto, Ana Paula Scaramal Ferré Manzanero, Juan 2018 A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal Toxins 10 5 193 210
dc.subject Proteïnes
dc.subject Insecticides
dc.title A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal
dc.type info:eu-repo/semantics/article
dc.date.updated 2018-11-19T14:43:39Z
dc.identifier.doi https://doi.org/10.3390/toxins10050193
dc.identifier.idgrec 126257

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