Effect of the high molecular weight chitosan and sodium alginate on Candida albicans hydrophobicity and adhesion to cells
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Effect of the high molecular weight chitosan and sodium alginate on Candida albicans hydrophobicity and adhesion to cells

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Effect of the high molecular weight chitosan and sodium alginate on Candida albicans hydrophobicity and adhesion to cells

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dc.contributor.author Azcurra, Ana Isabel es
dc.contributor.author Barembaum, Silvina Ruth es
dc.contributor.author Bojanich, Alejandra es
dc.contributor.author Calamari, Silvia Edith es
dc.contributor.author Aguilar, Javier es
dc.contributor.author Battellino, Luis José es
dc.contributor.author Dorronsoro de Cattoni, Susana es
dc.date.accessioned 2017-12-13T08:24:58Z
dc.date.available 2017-12-13T08:24:58Z
dc.date.issued 2006 es
dc.identifier.uri http://hdl.handle.net/10550/63538
dc.description.abstract The aim of the present paper is to evaluate the effect of the high molecular weight chitosan (HMWC) and of sodium alginate (NaAL) on surface hydrophobicity of Candida albicans and on adhesion of the yeast to epithelial cells and fibroblasts of different proceeding. For this study, a collection strain and seven isolates of C. albicans from saliva (patients with denture stomatitis) were grown in Sabouraud glucose agar supplemented with HMWC or NaAL or in absence of them (control). Hydrophobicity was determined by adhesion to hydrocarbons method using two organic media (xylene and chloroform). For adhesion experiments, aqueous suspensions of yeasts were contacted with solutions of biopolymers and different cells (rat and human fibroblasts and epithelial cells Hep-2). The quantification of adhesion was made by optical microscopy. Results: a decrease in hydrophobicity was observed in the presence of HMWC (44%) and of NaAL (82%) when chloroform was employed as organic medium, meanwhile the decreases were of 30% with HMWC and 19% with NaAL in the presence of xylene. Adhesion of C. albicans to epithelial cells and human fibroblasts decreased significantly with both biopolymers. In the case of rat fibroblasts, a decrease was observed only with NaAL. None of experiments showed significant differences associated to fibroblast type. Conclusions: biopolymers showed effectiveness in reducing hydrophobicity and adhesion of C. albicans to cells, which are important virulence factors related to colonization of the soft tissues of host or acrylic surfaces present in the oral system. es
dc.source Azcurra, Ana Isabel ; Barembaum, Silvina Ruth ; Bojanich, Alejandra ; Calamari, Silvia Edith ; Aguilar, Javier ; Battellino, Luis José ; Dorronsoro de Cattoni, Susana. Effect of the high molecular weight chitosan and sodium alginate on Candida albicans hydrophobicity and adhesion to cells. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 11 2 2006: 5- es
dc.title Effect of the high molecular weight chitosan and sodium alginate on Candida albicans hydrophobicity and adhesion to cells es
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi es

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