Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit
NAGIOS: RODERIC FUNCIONANDO

Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit

Repositori DSpace/Manakin

Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit

Mostra el registre complet de l'element

Visualització       (8.162Mb)

Exportar a Refworks
    
Bañó Polo, Manuel; Martínez Garay, Carlos Andrés; Grau, Brayan; Martínez Gil, Luis; Mingarro Muñoz, Ismael Perfil
Aquest document és un/a article, creat/da en: 2017
Translocon-associated protein (TRAP) complex is intimately associated with the ER translocon for the insertion or translocation of newly synthesised proteins in eukaryotic cells. The TRAP complex is comprised of three single-spanning and one multiple-spanning subunits. We have investigated the membrane insertion and topology of the multiple-spanning TRAP-γ subunit by glycosylation mapping and green fluorescent protein fusions both in vitro and in cell cultures. Results demonstrate that TRAP-γ has four transmembrane (TM) segments, an Nt/Ct cytosolic orientation and that the less hydrophobic TM segment inserts efficiently into the membrane only in the cellular context of full-length protein.

    Bañó Polo, Manuel Martínez Garay, Carlos Andrés Grau, Brayan Martínez Gil, Luis Mingarro Muñoz, Ismael 2017 Membrane insertion and topology of the translocon-associated protein (TRAP) gamma subunit Biochimica et Biophysica Acta-Biomembranes 1859 5 903 909
http://dx.doi.org/10.1016/j.bbamem.2017.01.027
distribuït sota llicència Creative Commons de Reconeixement-NoComercial 3.0 No adaptada

Aquest element apareix en la col·lecció o col·leccions següent(s)

Mostra el registre complet de l'element

Cerca a RODERIC

Cerca avançada

Visualitza

Estadístiques