RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins
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RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins

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RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins

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dc.contributor.author Navarro Bohigues, José Antonio
dc.contributor.author Genovés, A.
dc.contributor.author Climent, J.
dc.contributor.author Saurí Peris, Ana
dc.contributor.author Martínez Gil, Luis
dc.contributor.author Mingarro Muñoz, Ismael
dc.contributor.author Pallás Benet, Vicente
dc.date.accessioned 2016-11-30T16:08:53Z
dc.date.available 2016-11-30T16:08:53Z
dc.date.issued 2006
dc.identifier.uri http://hdl.handle.net/10550/56213
dc.description.abstract Advances in structural and biochemical properties of carmovirus movement proteins (MPs) have only been obtained in p7 and p9 from Carnation mottle virus (CarMV). Alignment of carmovirus MPs revealed a low conservation of amino acid identity but interestingly, similarity was elevated in regions associated with the functional secondary structure elements reported for CarMV which were conserved in all studied proteins. Nevertheless, some differential features in relation with CarMV MPs were identified in those from Melon necrotic virus (MNSV) (p7A and p7B). p7A was a soluble non-sequence specific RNA-binding protein, but unlike CarMV p7, its central region alone could not account for the RNA-binding properties of the entire protein. In fact, a 22-amino acid synthetic peptide whose sequence corresponds to this central region rendered an apparent dissociation constant (K(d)) significantly higher than that of the corresponding entire protein (9 mM vs. 0.83-25.7 microM). This p7A-derived peptide could be induced to fold into an alpha-helical structure as demonstrated for other carmovirus p7-like proteins. Additionally, in vitro fractionation of p7B transcription/translation mixtures in the presence of ER-derived microsomal membranes strongly suggested that p7B is an integral membrane protein. Both characteristics of these two small MPs forming the double gene block (DGB) of MNSV are discussed in the context of the intra- and intercellular movement of carmovirus.
dc.language.iso eng
dc.relation.ispartof Virology, 2006, vol. 356, num. 1, p. 57-67
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Navarro Bohigues, José Antonio Genovés, A. Climent, J. Saurí Peris, Ana Martínez Gil, Luis Mingarro Muñoz, Ismael Pallás Benet, Vicente 2006 RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins Virology 356 1 57 67
dc.subject Proteïnes de membrana
dc.subject Membranes (Biologia)
dc.subject Virus
dc.title RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-11-30T16:08:53Z
dc.identifier.doi http://dx.doi.org/10.1016/j.virol.2006.07.040
dc.identifier.idgrec 033788

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