Amylases from orange leaves. Characterization and relation to starch breakdown.
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Amylases from orange leaves. Characterization and relation to starch breakdown.

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Amylases from orange leaves. Characterization and relation to starch breakdown.

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Sanz Grau, Amparo; Guardiola Bárcena, José Luis
Aquest document és un/a article, creat/da en: 1988
Starch content in adult orange (Citrus sinensis L. Osbeck) leaves was highest at the end of the winter rest period and decreased during flowering and fruit set. Young inflorescence leaves accumulated starch until the June drop period to decrease to a low value at the end of it. Both, α- and ß-amylase activities were found in the leaves and the enzymes separated through Sephadex G100 filtration. By electrophoresis in polyacrylamide gels with 0.25 % amylopectine, α-amylase was resolved in 5 bands, and ß-amylase in 7. Total amylolitic activity in the leaves was determined throughout the annual cycle in both adult and young leaves. Enzyme activities were not correlated with the rate of starch breakdown, so a regulatory rôle for α-amylase in starch mobilization in the leaves is not apparent.

    Sanz Grau, Amparo Guardiola Bárcena, José Luis 1988 Amylases from orange leaves. Characterization and relation to starch breakdown. Plant Physiology (Life Sci. Adv.) 7 85 89
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