Insertion and topology of a plant viral movement protein in the Endoplasmic Reticulum membrane
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Insertion and topology of a plant viral movement protein in the Endoplasmic Reticulum membrane

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Insertion and topology of a plant viral movement protein in the Endoplasmic Reticulum membrane

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dc.contributor.author Vilar, Marçal
dc.contributor.author Saurí Peris, Ana
dc.contributor.author Monné, Magnus
dc.contributor.author Marcos López, José Francisco
dc.contributor.author Heijne, Gunnar von
dc.contributor.author Pérez Payá, Enrique
dc.contributor.author Mingarro Muñoz, Ismael
dc.date.accessioned 2016-09-08T14:39:02Z
dc.date.available 2016-09-08T14:39:02Z
dc.date.issued 2002
dc.identifier.uri http://hdl.handle.net/10550/54969
dc.description.abstract Virus-encoded movement proteins (MPs) mediate cell-to-cell spread of viral RNA through plant membranous intercellular connections, the plasmodesmata. The molecular pathway by which MPs interact with viral genomes and target plasmodesmata channels is largely unknown. The 9-kDa MP from carnation mottle carmovirus (CarMV) contains two potential transmembrane domains. To explore the possibility that this protein is in fact an intrinsic membrane protein, we have investigated its insertion into the endoplasmic reticulum membrane. By using in vitro translation in the presence of dog pancreas microsomes, we demonstrate that CarMV p9 inserts into the endoplasmic reticulum without the aid of any additional viral or plant host components. We further show that the membrane topology of CarMV p9 is N(cyt)-C(cyt) (N and C termini of the protein facing the cytoplasm) by in vitro translation of a series of truncated and full-length constructs with engineered glycosylation sites. Based on these results, we propose a topological model in which CarMV p9 is anchored in the membrane with its N- and C-terminal tail segments interacting with its soluble, RNA-bound partner CarMV p7, to accomplish the viral cell-to-cell movement function.
dc.language.iso eng
dc.relation.ispartof Journal of Biological Chemistry, 2002, vol. 277, num. 26, p. 23447-23452
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Vilar, Marçal Saurí Peris, Ana Monné, Magnus Marcos López, José Francisco Heijne, Gunnar von Pérez Payá, Enrique Mingarro Muñoz, Ismael 2002 Insertion and topology of a plant viral movement protein in the Endoplasmic Reticulum membrane Journal of Biological Chemistry 277 26 23447 23452
dc.subject Proteïnes de membrana
dc.subject Virus
dc.subject Bioquímica
dc.title Insertion and topology of a plant viral movement protein in the Endoplasmic Reticulum membrane
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-09-08T14:39:02Z
dc.identifier.doi http://dx.doi.org/10.1074/jbc.M202935200
dc.identifier.idgrec 003981

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