Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow
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Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow

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Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow

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dc.contributor.author Zheng, Junke
dc.contributor.author Lu, Zhigang
dc.contributor.author Kocabas, Fatih
dc.contributor.author Böttcher, Ralph T.
dc.contributor.author Costell Rosselló, Mercedes
dc.contributor.author Kang, Xunlei
dc.contributor.author Liu, Xiaoye
dc.contributor.author DeBerardinis, Ralph J.
dc.contributor.author Wang, Qianming
dc.contributor.author Chen, Guo-Qiang
dc.contributor.author Sadek, Hesham
dc.contributor.author Zhang, Cheng Cheng
dc.date.accessioned 2016-07-20T15:00:34Z
dc.date.available 2016-07-20T15:00:34Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10550/54642
dc.description.abstract How stem cells interact with the microenvironment to regulate their cell fates and metabolism is largely unknown. Here we demonstrated that the deletion of the cytoskeleton-modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in vivo. A switch from glycolysis to mitochondrial respiration with increased reactive oxygen species (ROS) level was also observed in HSCs on pfn1 deletion. Importantly, treatment of pfn1-deficient mice with the antioxidant N-acetyl-l-cysteine reversed the ROS level and loss of quiescence of HSCs, suggesting that the metabolism is mechanistically linked to the cell cycle quiescence of stem cells. The actin-binding and proline-binding activities of pfn1 are required for its function in HSCs. Our study provided evidence that pfn1 at least partially acts through the axis of pfn1/Gα13/EGR1 to regulate stem cell retention and metabolism in the bone marrow.
dc.language.iso eng
dc.relation.ispartof Blood, 2014, vol. 123, num. 7, p. 992-1001
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Zheng, Junke Lu, Zhigang Kocabas, Fatih Böttcher, Ralph T. Costell Rosselló, Mercedes Kang, Xunlei Liu, Xiaoye DeBerardinis, Ralph J. Wang, Qianming Chen, Guo-Qiang Sadek, Hesham Zhang, Cheng Cheng 2014 Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow Blood 123 7 992 1001
dc.subject Cèl·lules mare
dc.subject Proteïnes
dc.title Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-07-20T15:00:34Z
dc.identifier.doi http://dx.doi.org/10.1182/blood-2013-04-498469
dc.identifier.idgrec 111996

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