Novel mutations in the voltage-gated sodium channel of pyrethroid-resistant Varroa destructor populations from the Southeastern USA
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Novel mutations in the voltage-gated sodium channel of pyrethroid-resistant Varroa destructor populations from the Southeastern USA

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Novel mutations in the voltage-gated sodium channel of pyrethroid-resistant Varroa destructor populations from the Southeastern USA

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dc.contributor.author González Cabrera, Joel
dc.contributor.author Rodríguez Vargas, Sonia
dc.contributor.author Emyr Davies, T. G.
dc.contributor.author Field, Linda M.
dc.contributor.author Schmehl, Daniel
dc.contributor.author Ellis, James D.
dc.contributor.author Krieger, Klemens
dc.contributor.author Williamson, Martin S.
dc.date.accessioned 2016-07-05T17:52:22Z
dc.date.available 2016-07-05T17:52:22Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/10550/54403
dc.description.abstract The parasitic mite Varroa destructor has a significant worldwide impact on bee colony health. In the absence of control measures, parasitized colonies invariably collapse within 3 years. The synthetic pyrethroids tau-fluvalinate and flumethrin have proven very effective at managing this mite within apiaries, but intensive control programs based mainly on one active ingredient have led to many reports of pyrethroid resistance. In Europe, a modification of leucine to valine at position 925 (L925V) of the V. destructor voltage-gated sodium channel was correlated with resistance, the mutation being found at high frequency exclusively in hives with a recent history of pyrethroid treatment. Here, we identify two novel mutations, L925M and L925I, in tau-fluvalinate resistant V. destructor collected at seven sites across Florida and Georgia in the Southeastern region of the USA. Using a multiplexed TaqMan® allelic discrimination assay, these mutations were found to be present in 98% of the mites surviving tau-fluvalinate treatment. The mutations were also found in 45% of the non-treated mites, suggesting a high potential for resistance evolution if selection pressure is applied. The results from a more extensive monitoring programme, using the Taqman® assay described here, would clearly help beekeepers with their decision making as to when to include or exclude pyrethroid control products and thereby facilitate more effective mite management programmes.
dc.language.iso eng
dc.relation.ispartof Plos One, 2016, vol. 11, num. 5, p. e0155332
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source González Cabrera, Joel Rodríguez Vargas, Sonia Emyr Davies, T. G. Field, Linda M. Schmehl, Daniel Ellis, James D. Krieger, Klemens Williamson, Martin S. 2016 Novel mutations in the voltage-gated sodium channel of pyrethroid-resistant Varroa destructor populations from the Southeastern USA Plos One 11 5 e0155332
dc.subject Àcars
dc.subject Abelles
dc.subject Paràsits
dc.subject Microbiologia
dc.title Novel mutations in the voltage-gated sodium channel of pyrethroid-resistant Varroa destructor populations from the Southeastern USA
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-07-05T17:52:22Z
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0155332
dc.identifier.idgrec 113388

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