The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons
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The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons

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The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons

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dc.contributor.author García Martínez, José
dc.contributor.author Delgado Ramos, Lidia
dc.contributor.author Ayala Gallego, Guillermo
dc.contributor.author Pelechano García, Vicente José
dc.contributor.author Medina Salas, Daniel Alejandro
dc.contributor.author Carrasco, Fany
dc.contributor.author González, Ramón
dc.contributor.author Andrés-León, Eduardo
dc.contributor.author Steinmetz, Lars
dc.contributor.author Warringer, Jonas
dc.contributor.author Chávez, Sebastián
dc.contributor.author Pérez Ortín, José Enrique
dc.date.accessioned 2016-03-08T13:27:32Z
dc.date.available 2016-03-08T13:27:32Z
dc.date.issued 2015
dc.identifier.uri http://hdl.handle.net/10550/51801
dc.description.abstract We analyzed 80 different genomic experiments, and found a positive correlation between both RNA polymerase II transcription and mRNA degradation with growth rates in yeast. Thus, in spite of the marked variation in mRNA turnover, the total mRNA concentration remained approximately constant. Some genes, however, regulated their mRNA concentration by uncoupling mRNA stability from the transcription rate. Ribosome-related genes modulated their transcription rates to increase mRNA levels under fast growth. In contrast, mitochondria-related and stress-induced genes lowered mRNA levels by reducing mRNA stability or the transcription rate, respectively. We also detected these regulations within the heterogeneity of a wild-type cell population growing in optimal conditions. The transcriptomic analysis of sorted microcolonies confirmed that the growth rate dictates alternative expression programs by modulating transcription and mRNA decay. The regulation of overall mRNA turnover keeps a constant ratio between mRNA decay and the dilution of [mRNA] caused by cellular growth. This regulation minimizes the indiscriminate transmission of mRNAs from mother to daughter cells, and favors the response capacity of the latter to physiological signals and environmental changes. We also conclude that, by uncoupling mRNA synthesis from decay, cells control the mRNA abundance of those gene regulons that characterize fast and slow growth.
dc.language.iso eng
dc.relation.ispartof Nucleic Acids Research, 2015, vol. 2015, p. 1-16
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source García Martínez, José Delgado Ramos, Lidia Ayala Gallego, Guillermo Pelechano García, Vicente José Medina Salas, Daniel A. Carrasco, Fany González, Ramón Andrés-León, Eduardo Steinmetz, Lars Warringer, Jonas Chávez, Sebastián Pérez Ortín, José Enrique 2015 The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons Nucleic Acids Research 2015 1 16
dc.subject Genètica molecular
dc.subject RNA
dc.subject Transcripció genètica
dc.title The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-03-08T13:27:32Z
dc.identifier.doi http://dx.doi.org/10.1093/nar/gkv1512
dc.identifier.idgrec 108979

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