Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species
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Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species

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Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species

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Hernández Rodríguez, Carmen Sara; Hernández Martínez, Patricia; Van Rie, Jeroen; Ferré Manzanero, Juan Perfil
This document is a artículoDate2012

Este documento está disponible también en : http://hdl.handle.net/10550/49196
Cry1Fa insecticidal protein was successfully radiolabeled with 125I-Na. Specific binding to brush border membrane vesicles was shown for the lepidopteran species Ostrinia nubilalis, Spodoptera frugiperda, Spodoptera exigua, Helicoverpa armigera, Heliothis virescens, and Plutella xylostella. Homologous competition assays were performed to obtain equilibrium binding parameters (Kd [dissociation constant] and Rt [concentration of binding sites]) for these six insect species.

    Hernández Rodríguez, Carmen Sara Hernández Martínez, Patricia J. Van Rie Van Rie, Jeroen Ferré Manzanero, Juan 2012 Specific binding of radiolabeled Cry1Fa insecticidal protein from Bacillus thuringiensis to midgut sites in lepidopteran species Applied and Environmental Microbiology 78 11 4048 4050
http://dx.doi.org/10.1128/AEM.07591-11

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