Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes
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Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes

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Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes

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dc.contributor.author Platas Gil, Julia es_ES
dc.contributor.author Guillén Salazar, María Isabel es_ES
dc.contributor.author Pérez del Caz, María Dolores es_ES
dc.contributor.author Gomar Sancho, Francisco es_ES
dc.contributor.author Mirabet, Vicente es_ES
dc.contributor.author Alcaraz Tormo, María José es_ES
dc.date.accessioned 2015-06-18T12:29:22Z
dc.date.available 2015-06-18T12:29:22Z
dc.date.issued 2013 es_ES
dc.identifier.uri http://hdl.handle.net/10550/44481
dc.description.abstract Osteoarthritis (OA) is the most frequent joint disorder and an important cause of disability. Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair. We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA chondrocytes a number of key mediators involved in cartilage degeneration. CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. In addition, CM increased IL-10 levels and counteracted the stimulating effects of IL-1β on the production of tumor necrosis factor-α, IL-6, prostaglandin E2, and NO measured as nitrite and the mRNA expression of these cytokines, CCL-2, CCL-3, CCL-4, CCL-5, CCL-8, CCL-19, CCL-20, CXCL-1, CXCL-2, CXCL-3, CXCL-5, CXCL-8, cyclooxygenase-2, microsomal prostaglandin E synthase-1, and inducible NO synthase. These effects may be dependent on the inhibition of nuclear factor-κB activation by CM. Our data demonstrate the chondroprotective actions of CM and provide support for further studies of this approach in joint disease. es_ES
dc.source Mediators of Inflammation Vol. 2013 es_ES
dc.title Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.identifier.doi 10.1155/2013/357014 es_ES
dc.identifier.idgrec 091720 es_ES

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