PTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations
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PTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations

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PTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations

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dc.contributor.author Inglés de la Torre, Marta es_ES
dc.contributor.author Gambini Ricapa, Juan es_ES
dc.contributor.author Miguel, M. Graça es_ES
dc.contributor.author Bonet Costa, Vicent es_ES
dc.contributor.author Abdelaziz, Kheira M. es_ES
dc.contributor.author El Alami, Marya es_ES
dc.contributor.author Viña Ribes, José es_ES
dc.contributor.author Borrás Blasco, Consuelo es_ES
dc.date.accessioned 2015-06-05T12:40:31Z
dc.date.available 2015-06-05T12:40:31Z
dc.date.issued 2014 es_ES
dc.identifier.uri http://hdl.handle.net/10550/44194
dc.description.abstract Introduction. Antioxidant properties of resveratrol have been intensively studied for the last years, both in vivo and in vitro. Its bioavailability after an oral dose is very low and therefore it is very important to make sure that plasma concentrations of free resveratrol are sufficient enough to be active as antioxidant. Aims. In the present study, using nutritionally relevant concentrations of resveratrol, we aim to confirm its antioxidant capacity on reducing peroxide levels and look for the molecular pathway involved in this antioxidant effect. Methods. We used mammary gland tumor cells (MCF-7), which were pretreated with different concentrations of resveratrol for 48 h, and/or a PTEN inhibitor (bpV: bipy). Hydrogen peroxide levels were determined by fluorimetry, PTEN levels and Akt phosphorylation by Western Blotting, and mRNA expression of antioxidant genes by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. Resveratrol treatment for 48 h lowered peroxide levels in MCF-7, even at low nutritional concentrations (1 nM). This effect was mediated by the activation of PTEN/Akt pathway, which resulted in an upregulation of catalase and MnSOD mRNA levels. Conclusion. Resveratrol acts as an antioxidant at nutritionally relevant concentrations by inducing the expression of antioxidant enzymes, through a mechanism involving PTEN/Akt signaling pathway. es_ES
dc.source BioMed Research International Vol. 2014 es_ES
dc.title PTEN Mediates the Antioxidant Effect of Resveratrol at Nutritionally Relevant Concentrations es_ES
dc.type info:eu-repo/semantics/article
dc.identifier.doi 10.1155/2014/580852 es_ES
dc.identifier.idgrec 097497 es_ES

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