Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy
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Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy

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Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy

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Doblas Expósito, Ana Isabel; Sánchez Ortiga, Emilio; Saavedra Tortosa, Genaro; Martínez Corral, Manuel Perfil; García Sucerquia, Jorge Iván
Aquest document és un/a article, creat/da en: 2014
The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.

    Doblas Expósito, Ana Isabel Sánchez Ortiga, Emilio Saavedra Tortosa, Genaro Martínez Corral, Manuel García Sucerquia, Jorge I. 2014 Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy Journal of Biomedical Optics 19 4 046022
http://dx.doi.org/10.1117/1.JBO.19.4.046022
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