On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation
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On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation

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On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation

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dc.contributor.author Pérez Través, Laura
dc.contributor.author Lopes, Christian A.
dc.contributor.author Querol Simón, Amparo
dc.contributor.author Barrio Esparducer, Eladio
dc.date.accessioned 2014-11-03T09:26:50Z
dc.date.available 2014-11-03T09:26:50Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10550/39525
dc.description.abstract Although the genus Saccharomyces has been thoroughly studied, some species in the genus has not yet been accurately resolved; an example is S. bayanus, a taxon that includes genetically diverse lineages of pure and hybrid strains. This diversity makes the assignation and classification of strains belonging to this species unclear and controversial. They have been subdivided by some authors into two varieties (bayanus and uvarum), which have been raised to the species level by others. In this work, we evaluate the complexity of 46 different strains included in the S. bayanus taxon by means of PCR-RFLP analysis and by sequencing of 34 gene regions and one mitochondrial gene. Using the sequence data, and based on the S. bayanus var. bayanus reference strain NBRC 1948, a hypothetical pure S. bayanus was reconstructed for these genes that showed alleles with similarity values lower than 97% with the S. bayanus var. uvarum strain CBS 7001, and of 99¿100% with the non S. cerevisiae portion in S. pastorianus Weihenstephan 34/70 and with the new species S. eubayanus. Among the S. bayanus strains under study, different levels of homozygosity, hybridization and introgression were found; however, no pure S. bayanus var. bayanus strain was identified. These S. bayanus hybrids can be classified into two types: homozygous (type I) and heterozygous hybrids (type II), indicating that they have been originated by different hybridization processes. Therefore, a putative evolutionary scenario involving two different hybridization events between a S. bayanus var. uvarum and unknown European S. eubayanus-like strains can be postulated to explain the genomic diversity observed in our S. bayanus var. bayanus strains.
dc.language.iso eng
dc.relation.ispartof Plos One, 2014, vol. 9, num. 4, p. e93729
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Pérez Través, Laura Lopes, Christian A. Querol Simón, Amparo Barrio Esparducer, Eladio 2014 On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation Plos One 9 4 e93729
dc.subject Genètica molecular
dc.subject Saccharomyces
dc.title On the complexity of the Saccharomyces bayanus taxon: hybridization and potential hybrid speciation
dc.type info:eu-repo/semantics/article
dc.date.updated 2014-11-03T09:26:50Z
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0093729
dc.identifier.idgrec 096142

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