The adult spinal cord harbors a population of GFAP-positive progenitors with limited self-renewal potential
NAGIOS: RODERIC FUNCIONANDO

The adult spinal cord harbors a population of GFAP-positive progenitors with limited self-renewal potential

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The adult spinal cord harbors a population of GFAP-positive progenitors with limited self-renewal potential

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dc.contributor.author Fiorelli, R.
dc.contributor.author Cebrián Silla, Arantxa
dc.contributor.author García Verdugo, José Manuel
dc.contributor.author Raineteau, O.
dc.date.accessioned 2014-05-12T11:02:01Z
dc.date.available 2014-05-12T11:02:01Z
dc.date.issued 2013
dc.identifier.uri http://dx.doi.org/10.1002/glia.22579
dc.identifier.uri http://hdl.handle.net/10550/35038
dc.description.abstract Adult neural stem cells (aNSCs) of the forebrain are GFAP-expressing cells that are intercalated within ependymal cells of the subventricular zone (SVZ). Cells showing NSCs characteristics in vitro can also be isolated from the periaqueductal region in the adult spinal cord (SC), but contradicting results exist concerning their glial versus ependymal identity. We used an induci- ble transgenic mouse line (hGFAP-CreERT2) to conditionally label GFAP-expressing cells in the adult SVZ and SC periaque- duct, and directly and systematically compared their self-renewal and multipotential properties in vitro. We demonstrate that a population of GFAP1 cells that share the morphology and the antigenic properties of SVZ-NSCs mostly reside in the dorsal aspect of the central canal (CC) throughout the spinal cord. These cells are non-proliferative in the intact spinal cord, but incorporate the S-phase marker EdU following spinal cord injury. Multipotent, clonal YFP-expressing neurospheres (i.e., deriv- ing from recombined GFAP-expressing cells) were successfully obtained from both the intact and injured spinal cord. These spheres however showed limited self-renewal properties when compared with SVZ-neurospheres, even after spinal cord injury. Altogether, these results demonstrate that significant differences exist in NSCs lineages between neurogenic and non- neurogenic regions of the adult CNS. Thus, although we confirm that a population of multipotent GFAP1 cells co-exists alongside with multipotent ependymal cells within the adult SC, we identify these cells as multipotent progenitors showing limited self-renewal properties.
dc.relation.ispartof Glia, 2013, vol. 61, num. 12, p. 2100-2113
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Fiorelli, R.; Cebrián Silla, Arantxa; Garcia Verdugo, Jose Manuel; Raineteau, O. (2013) The adult spinal cord harbors a population of GFAP-positive progenitors with limited self-renewal potential. Glia 61 12 2100 2113
dc.subject Xarxes neuronals (Neurobiologia)
dc.title The adult spinal cord harbors a population of GFAP-positive progenitors with limited self-renewal potential
dc.type info:eu-repo/semantics/article
dc.date.updated 2014-05-12T11:02:01Z
dc.identifier.doi http://dx.doi.org/10.1002/glia.22579
dc.identifier.idgrec 093146

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