Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing
NAGIOS: RODERIC FUNCIONANDO

Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing

DSpace Repository

Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing

Show simple item record

dc.contributor.author Bañó Polo, Manuel
dc.contributor.author Baeza Delgado, Carlos
dc.contributor.author Orzáez Calatayud, María del Mar
dc.contributor.author Martí Renom, Marc A.
dc.contributor.author Abad Mazario, Concepción
dc.contributor.author Mingarro Muñoz, Ismael
dc.date.accessioned 2014-03-26T12:40:20Z
dc.date.available 2014-03-26T12:40:20Z
dc.date.issued 2012
dc.identifier.uri http://dx.doi.org/10.1371/journal.pone.0044263
dc.identifier.uri http://hdl.handle.net/10550/33702
dc.description.abstract The vast majority of membrane proteins are anchored to biological membranes through hydrophobic α-helices. Sequence analysis of high-resolution membrane protein structures show that ionizable amino acid residues are present in transmembrane (TM) helices, often with a functional and/or structural role. Here, using as scaffold the hydrophobic TM domain of the model membrane protein glycophorin A (GpA), we address the consequences of replacing specific residues by ionizable amino acids on TM helix insertion and packing, both in detergent micelles and in biological membranes. Our findings demonstrate that ionizable residues are stably inserted in hydrophobic environments, and tolerated in the dimerization process when oriented toward the lipid face, emphasizing the complexity of protein-lipid interactions in biological membranes.
dc.relation.ispartof Plos One, 2012, vol. 7, num. 9, p. e44263
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Bañó Polo, Manuel Baeza Delgado, Carlos Orzáez Calatayud, María del Mar Marti Renom, Marc Abad Mazario, Concepción Mingarro Muñoz, Ismael 2012 Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing Plos One 7 9 e44263
dc.subject Membranes (Biologia)
dc.subject Biotecnologia
dc.title Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing
dc.type info:eu-repo/semantics/article
dc.date.updated 2014-03-26T12:40:21Z
dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0044263
dc.identifier.idgrec 079791

View       (713.4Kb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics