Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing
NAGIOS: RODERIC FUNCIONANDO

Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing

Repositori DSpace/Manakin

Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing

Mostra el registre complet de l'element

Visualització       (713.4Kb)

Exportar a Refworks
    
Bañó Polo, Manuel; Baeza Delgado, Carlos; Orzáez Calatayud, María del Mar; Martí Renom, Marc A.; Abad Mazario, Concepción; Mingarro Muñoz, Ismael Perfil
Aquest document és un/a article, creat/da en: 2012

Este documento está disponible también en : http://dx.doi.org/10.1371/journal.pone.0044263
The vast majority of membrane proteins are anchored to biological membranes through hydrophobic α-helices. Sequence analysis of high-resolution membrane protein structures show that ionizable amino acid residues are present in transmembrane (TM) helices, often with a functional and/or structural role. Here, using as scaffold the hydrophobic TM domain of the model membrane protein glycophorin A (GpA), we address the consequences of replacing specific residues by ionizable amino acids on TM helix insertion and packing, both in detergent micelles and in biological membranes. Our findings demonstrate that ionizable residues are stably inserted in hydrophobic environments, and tolerated in the dimerization process when oriented toward the lipid face, emphasizing the complexity of protein-lipid interactions in biological membranes.

    Bañó Polo, Manuel Baeza Delgado, Carlos Orzáez Calatayud, María del Mar Marti Renom, Marc Abad Mazario, Concepción Mingarro Muñoz, Ismael 2012 Polar/Ionizable Residues in Transmembrane Segments: Effects on Helix-Helix Packing Plos One 7 9 e44263
http://dx.doi.org/10.1371/journal.pone.0044263
distribuït sota llicència Creative Commons de Reconeixement-NoComercial 3.0 No adaptada

Aquest element apareix en la col·lecció o col·leccions següent(s)

Mostra el registre complet de l'element

Cerca a RODERIC

Cerca avançada

Visualitza

Estadístiques