Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
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Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii

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Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii

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dc.contributor.author Belloch, Carmela
dc.contributor.author Pérez Torrado, Roberto
dc.contributor.author González, Sara S.
dc.contributor.author Pérez Ortín, José Enrique
dc.contributor.author García Martínez, José
dc.contributor.author Querol Simón, Amparo
dc.contributor.author Barrio Esparducer, Eladio
dc.date.accessioned 2013-12-02T13:23:07Z
dc.date.available 2013-12-02T13:23:07Z
dc.date.issued 2009
dc.identifier.uri http://dx.doi.org/10.1128/AEM.02282-08
dc.identifier.uri http://hdl.handle.net/10550/31687
dc.description.abstract Recently, a new type of hybrid resulting from the hybridization between Saccharomyces cerevisiae and Saccharomyces kudriavzevii was described. These strains exhibit physiological properties of potential biotechnological interest. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. We characterized the genomic constitution of several wine S. cerevisiae × S. kudriavzevii strains by using a combined approach based on the restriction fragment length polymorphism analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis, and gene dose determination by quantitative real-time PCR. The high similarity in the genome structures of the S. cerevisiae × S. kudriavzevii hybrids under study indicates that they originated from a single hybridization event. After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimeric chromosomes by the nonreciprocal recombination between homeologous chromosomes. These nonreciprocal recombinations between homeologous chromosomes occurred in highly conserved regions, such as Ty long terminal repeats (LTRs), rRNA regions, and conserved protein-coding genes. This study supports the hypothesis that chimeric chromosomes may have been generated by a mechanism similar to the recombination-mediated chromosome loss acting during meiosis in Saccharomyces hybrids. As a result of the selective processes acting during fermentation, hybrid genomes maintained the S. cerevisiae genome but reduced the S. kudriavzevii fraction.
dc.relation.ispartof Applied and Environmental Microbiology, 2009, vol. 75, num. 8, p. 2534-2544
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Belloch, Carmela Pérez Torrado, Roberto González, Sara S. Pérez Ortín, José Enrique García Martínez, José Querol Simón, Amparo Barrio Esparducer, Eladio 2009 Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii Applied and Environmental Microbiology 75 8 2534 2544
dc.subject Genètica molecular
dc.subject Saccharomyces
dc.subject Saccharomyces cerevisiae
dc.title Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii
dc.type info:eu-repo/semantics/article
dc.date.updated 2013-12-02T13:23:07Z
dc.identifier.doi http://dx.doi.org/10.1128/AEM.02282-08
dc.identifier.idgrec 048855

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