Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.
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Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.

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Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.

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dc.contributor.author Romero Santacreu, Lorena
dc.contributor.author Moreno Mariño, Joaquín
dc.contributor.author Pérez Ortín, José Enrique
dc.contributor.author Alepuz Martínez, Paula
dc.date.accessioned 2013-11-30T13:08:16Z
dc.date.available 2013-11-30T13:08:16Z
dc.date.issued 2009
dc.identifier.uri http://dx.doi.org/10.1261/rna.1435709
dc.identifier.uri http://hdl.handle.net/10550/31673
dc.description.abstract Hyperosmotic stress yields reprogramming of gene expression in Saccharomyces cerevisiae cells. Most of this response is orchestrated by Hog1, a stress-activated, mitogen-activated protein kinase (MAPK) homologous to human p38. We investigated, on a genomic scale, the contribution of changes in transcription rates and mRNA stabilities to the modulation of mRNA amounts during the response to osmotic stress in wild-type and hog1 mutant cells. Mild osmotic shock induces a broad mRNA destabilization; however, osmo-mRNAs are up-regulated by increasing both transcription rates and mRNA half-lives. In contrast, mild or severe osmotic stress in hog1 mutants, or severe osmotic stress in wild-type cells, yields global mRNA stabilization and sequestration of mRNAs into P-bodies. After adaptation, the absence of Hog1 affects the kinetics of P-bodies disassembly and the return of mRNAs to translation. Our results indicate that regulation of mRNA turnover contributes to coordinate gene expression upon osmotic stress, and that there are both specific and global controls of mRNA stability depending on the strength of the osmotic stress.
dc.relation.ispartof RNA-A Publication of the Rna Society, 2009, vol. 15, num. 6, p. 1110-1120
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Romero Santacreu, Lorena Moreno Mariño, Joaquín Pérez Ortín, José Enrique Alepuz Martínez, Paula 2009 Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae. RNA-A Publication of the Rna Society 15 6 1110 1120
dc.subject Saccharomyces cerevisiae
dc.subject Genètica molecular
dc.subject Bioquímica
dc.title Specific and global regulation of mRNA stability during osmotic stress in Saccharomyces cerevisiae.
dc.type info:eu-repo/semantics/article
dc.date.updated 2013-11-30T13:08:16Z
dc.identifier.doi http://dx.doi.org/10.1261/rna.1435709
dc.identifier.idgrec 049949

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