Aging enhances contraction to thromboxane A2 in aorta from female senescence mice
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Aging enhances contraction to thromboxane A2 in aorta from female senescence mice

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Aging enhances contraction to thromboxane A2 in aorta from female senescence mice

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dc.contributor.author Novella del Campo, Susana
dc.contributor.author Dantas, A. P.
dc.contributor.author Segarra Irles, Gloria Vicenta
dc.contributor.author Novensá, L.
dc.contributor.author Bueno, C.
dc.contributor.author Heras, Magda
dc.contributor.author Hermenegildo Caudevilla, Carlos
dc.contributor.author Medina Bessó, Pascual
dc.date.accessioned 2013-11-18T11:42:52Z
dc.date.available 2013-11-18T11:42:52Z
dc.date.issued 2013
dc.identifier.uri http://dx.doi.org/10.1007/s11357-011-9337-y
dc.identifier.uri http://hdl.handle.net/10550/31106
dc.description.abstract The time-course for aging-associated effects on vascular reactivity to U46619, a stable analogue of thromboxane A2 (TXA2), was studied in aorta from female senescence-accelerated mice-prone (SAMP8), a murine model of accelerated senescence. SAMP8 and senescence-accelerated mice-resistant (SAMR1) were divided into three groups: 3-, 6- and 10-month-old. Contractile curves to U46619 (10−9 to 10−6 M) were performed in aortic rings in the absence or in the presence of nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME; 10−4 M) and/or cyclooxygenase (COX) inhibitor indomethacin (10−5 M). Protein and gene expression for COX-1 and COX-2 were determined by immunofluorescence and real-time PCR, respectively. Maximal contraction to U46619 was markedly higher in SAMP8 at all ages. In SAMR1, increases were seen at 10 months, while SAMP8 displays augmented contraction at 6 months, which was further increased at 10 months. L-NAME enhanced U46619 contractions in both 6-month-old groups, although the increase was higher on vessels from SAMR1 at this age. Indomethacin equally increased U46619 contractions in both 3-month-old groups, suggesting the production of vasodilator prostaglandin in young animals. In contrast, at 6 and 10 months indomethacin decreased U46619 contractions in both groups, indicating an aging-associated swap to a release of contractile prostanoids in aorta. In conclusion, aging enhances contractile responses to TXA2 in aorta from female mice by a mechanism involving a decrease of NO production and increased action of contractile prostanoids. This process occurs earlier in SAMP8 mice, establishing these mice as good model to study cardiovascular aging in a convenient and standard time-course.
dc.relation.ispartof Age, 2013, vol. 35, p. 117-128
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Novella del Campo, Susana; Dantas, A. P.; Segarra Irles, Gloria; Novensá, L.; Bueno, C.; Heras, M.; Hermenegildo Caudevilla, Carlos; Medina Bessó, Pascual (2013) Aging enhances contraction to thromboxane A2 in aorta from female senescence mice. Age 35 117 128
dc.subject Farmacologia
dc.subject Medicina
dc.title Aging enhances contraction to thromboxane A2 in aorta from female senescence mice
dc.type info:eu-repo/semantics/article
dc.date.updated 2013-11-18T11:42:52Z
dc.identifier.doi http://dx.doi.org/10.1007/s11357-011-9337-y
dc.identifier.idgrec 084861

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