Estudios estratégicos para la lucha contra la fascioliasis humana a nivel mundial = Strategic studies for the fight against de human fascioliasis worldwide
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Estudios estratégicos para la lucha contra la fascioliasis humana a nivel mundial = Strategic studies for the fight against de human fascioliasis worldwide

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Estudios estratégicos para la lucha contra la fascioliasis humana a nivel mundial = Strategic studies for the fight against de human fascioliasis worldwide

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dc.contributor.advisor Mas-Coma, S.
dc.contributor.advisor Valero Aleixandre, María Adela
dc.contributor.author Pérez Crespo, Ignacio
dc.contributor.other Departament de Biologia Cel.lular i Parasitologia es_ES
dc.date.accessioned 2013-09-13T10:52:19Z
dc.date.available 2013-10-14T06:10:03Z
dc.date.issued 2013
dc.date.submitted 13-09-2013 es_ES
dc.identifier.uri http://hdl.handle.net/10550/30015
dc.description.abstract Fascioliasis is a zoonotic parasitic disease caused by Fasciola hepatica and F. gigantica. Emergence of human fascioliasis prompted a worldwide control initiative including a pilot study in several countries. Given the necessity to characterize the fasciolid populations involved, the overall aim of the present research is, first, the phenotypic characterization of faciolid adults and eggs implicated in human endemic areas, where this initiative has been implemented, through a computer image analysis system (CIAS) applied on the basis of standardized measurements. Second, new immunological techniques and their potential use as immunological diagnostic tests in those areas are evaluated. The specific results have been grouped into five parts. A) Characterization of eggs of both F. hepatica and F. gigantica for their differential diagnosis. The study revealed that eggs shed by humans show morphological traits different from eggs shed by animals. In humans, F. hepatica eggs are bigger and F. gigantica eggs are smaller than reported to date from livestock, and their measurements overlap when compared. B) Study of the correlation between egg-shedding and uterus development in F. hepatica human and animal isolates. Uterus area (UA) development of adult F. hepatica obtained at different days post infection (dpi) in a Wistar rat model with isolates obtained from cattle, sheep, pigs and humans from the endemic human fascioliasis zone of the Northern Bolivian Altiplano was analysed and compared with the number of eggs shed per gram of faeces as obtained through the Kato–Katz technique. This work shows a positive correlation between liver fluke UA and egg production. C) Study of F. hepatica phenotypic characterization in Andean human endemic areas. The results showed that two phenotypic patterns could be distinguished in F. hepatica adult size: the valley pattern (Cajamarca and Mantaro, Peru) and the altiplanic pattern (northern Altiplano, Bolivia). Furthermore, the Andean valley population and European standard populations presented phenotypic homogeneity. The Altiplano population showed a large size range with a pronouncedly lower minimum size, indicating that uterus gravidity is reached at a smaller size than in valley populations. D) Study of the DRG Fasciola hepatica IgG ELISA test as a serological diagnostic tool of human fascioliasis in different epidemiological situations. The study shows that sensitivity and specificity of the DRG assay were 95.3% (95% confidence intervals, 82,9–99,2%) and 95,7% (95% confidence intervals, 92,3–97,5%), respectively. No correlation between egg output and the optical density (OD) values of the test was observed. E) Field evaluation of the MM3 coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries. As part of the above-mentioned control initiative, two hyperendemic areas were chosen: Huacullani, Northern Altiplano, Bolivia, representing the Altiplanic transmission pattern with high prevalences and intensities; the Cajamarca valley, Peru, representing the valley pattern with high prevalences but low intensities. It can be concluded that the coproantigen-detection test allows for high sensitivity and specificity, fast mass screening, detection in the chronic phase, early detection of treatment failure or reinfection in post-treated subjects, and is convenient in surveillance programmes. However, this technique falls short when evaluating the fluke burden on its own. es_ES
dc.format.extent 209 p. es_ES
dc.language.iso es es_ES
dc.subject natural population es_ES
dc.subject uterus es_ES
dc.subject wistar es_ES
dc.subject human es_ES
dc.subject egg es_ES
dc.subject sheep es_ES
dc.subject cattle es_ES
dc.subject F. gigantica es_ES
dc.subject pig es_ES
dc.subject bolivian altiplano es_ES
dc.subject number per gram of faeces es_ES
dc.subject cajamarca valley es_ES
dc.subject mantaro valley es_ES
dc.subject biometry es_ES
dc.subject peru es_ES
dc.subject bolivia es_ES
dc.subject adult es_ES
dc.subject georgia es_ES
dc.subject egypt es_ES
dc.subject growth model es_ES
dc.subject vietnam es_ES
dc.subject worldwide control initiative es_ES
dc.subject principal component analysis es_ES
dc.subject DRG Fasciola hepatica test es_ES
dc.subject MM3 coproantigen es_ES
dc.subject F. hepatica es_ES
dc.subject experimental population es_ES
dc.title Estudios estratégicos para la lucha contra la fascioliasis humana a nivel mundial = Strategic studies for the fight against de human fascioliasis worldwide es_ES
dc.type info:eu-repo/semantics/doctoralThesis es_ES
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA es_ES
dc.embargo.terms 1 month es_ES

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