Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione
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Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione

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Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione

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dc.contributor.author Zaragozá Colom, Rosa
dc.contributor.author García de Mier, Concepción
dc.contributor.author Rus, Ariana Diana
dc.contributor.author Pallardó, Federico V.
dc.contributor.author Barber Sanchis, Teresa
dc.contributor.author Torres Asensi, Luis
dc.contributor.author Miralles Fernández, Vicente
dc.contributor.author Viña Ribes, Juan
dc.date.accessioned 2010-08-10T10:52:36Z
dc.date.available 2010-08-10T10:52:36Z
dc.date.issued 2003
dc.identifier.uri http://hdl.handle.net/10550/16807
dc.description.abstract In the lactatingmammary gland, weaning produces mitochondrial cytochrome c release and nuclear DNA fragmentation, as determined by gel electrophoresis. This is followed by a significant decrease in lactation. Weaning for 2 h produces an early induction of the tumour suppressor/transcription factor p53, whereas the oncoprotein c-Jun and c-Jun N-terminal kinase are elevated after 24 h of weaning when compared with controls. The expression of p21cip1 and p27kip1, cyclin-dependent kinase inhibitors, was significantly higher in weaned rats when compared with control lactating rats. All the changes mentioned above also happen in the lactatingmammary gland when propargylglycine, an inhibitor of the liver trans-sulphuration pathway, is administered. This effect is partially reversed by N-acetylcysteine administration. The administration of buthionine sulphoximine, an irreversible inhibitor of γ -glutamylcysteine synthetase, to lactating rats produces a decrease in GSH levels and changes in protein concentrations and gene transcripts similar to those in rats with impaired trans-sulphuration pathway. These data suggest that the inter-tissue flux of GSH is an important mechanism of Lcysteine delivery to the lactating mammary gland and emphasize the importance of this physiological event inmaintaining the gene expression required to sustain lactation. en
dc.language.iso en en
dc.relation http://www.biochemj.org/bj/373/0825/3730825.pdf en
dc.source Zaragozá, R.; García, C.; Rus, A.D.; Pallardó, F.V.; Barber, T.; Torres, L.; Miralles, J.V.; Viña J.R. (2003). Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione. En Biochemical Journal, 373, 825–834 en
dc.subject apoptosis en
dc.subject γ-cystathionase en
dc.subject l-cysteine en
dc.subject glutathione (GSH) en
dc.subject lactation en
dc.title Inhibition of liver trans-sulphuration pathway by propargylglycine mimics gene expression changes found in the mammary gland of weaned lactating rats: role of glutathione en
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Bioquímica en
dc.identifier.doi https://doi.org/10.1042/BJ20030387
dc.description.private zacoro@uv.es; arus@alumni.uv.es; cgmier@uv.es; barber@uv.es; torresl@uv.es; mirallev@uv.es; vinaj@uv.es en
dc.identifier.idgrec 000650 en

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