Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected rats: kinetics of coproantigen excretion
NAGIOS: RODERIC FUNCIONANDO

Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected rats: kinetics of coproantigen excretion

DSpace Repository

Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected rats: kinetics of coproantigen excretion

Show simple item record

dc.contributor.author Toledo Navarro, Rafael
dc.contributor.author Espert Fernandez, Ana M.
dc.contributor.author Muñoz Antoli-Candela, Carla Teresa
dc.contributor.author Marcilla Diaz, Antonio
dc.contributor.author Fried, Bernard
dc.contributor.author Esteban Sanchis, Jose Guillermo
dc.date.accessioned 2010-08-09T10:39:12Z
dc.date.available 2010-08-09T10:39:12Z
dc.date.issued 2003
dc.identifier.uri http://hdl.handle.net/10550/16778
dc.description.abstract The present study reports on the development of a coproantigen capture enzyme-linked immunosorbent assay (ELISA) for detecting Echinostoma caproni in experimentally infected rats. The capture ELISA was based on polyclonal rabbit antibodies that recognize excretory–secretory (ES) antigens. The detection limit of pure ES was 3 ng/ml in sample buffer and 60 ng/ml in fecal samples. The test was evaluated using a follow-up of 10 rats experimentally infected with 100 metacercariae of E. caproni, and the results were compared with those of other diagnostic methods such as parasitological examination and antibody titers determined by indirect ELISA. Coproantigens were detected in all the infected rats from the first day postinfection (DPI). The period of maximal coproantigen excretion was between 7 and 21 DPI. The values remained positive until 49–56 DPI, coinciding with the disappearance of the eggs in the stool samples of the infected rats. The kinetics of coproantigen detection were correlated with those of egg output. The present assay provides an alternative tool for the diagnosis of the echinostome infections. The proposed capture ELISA makes possible an earlier diagnosis than that provided by parasitological examination and indirect ELISA and also allows for the differentiation of past and current infections. Our results show that this assay can also be used to monitor the course of echinostome infections. en
dc.language.iso en en
dc.relation http://www.journalofparasitology.org/doi/pdf/10.1645/GE-3191 en
dc.source TOLEDO NAVARRO, Rafael ; Espert Fernandez, Ana M. ; Muñoz Antoli-Candela, Carla Teresa ; Marcilla Diaz, Antonio ; Fried, Bernard ; Esteban Sanchis, Jose Guillermo, 2003, Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected rats: kinetics of coproantigen excretion, Journal of Parasitology, vol. 89, no. 3, p. 1227–1231 en
dc.subject Antibody ; Echinostoma caproni ; Infected rats ; Coproantigen Excretion ; Inmunosorbent en
dc.title Development of an antibody-based capture enzyme-linked immunosorbent assay for detecting echinostoma caproni (trematoda) in experimentally infected rats: kinetics of coproantigen excretion en
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Biología animal (Zoología) ::Parasitología animal en
dc.description.private Toledo Navarro, Rafael, Rafael.Toledo@uv.es ; Espert Fernandez, Ana M., Ana.M.Espert@uv.es ; Marcilla Diaz, Antonio, Antonio.Marcilla@uv.es ; Esteban Sanchis, Jose Guillermo, Jguillermo.Esteban@uv.es en
dc.identifier.idgrec 002446 en

View       (84.99Kb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics