Id2 leaves the chromatin of the E2F4–p130-controlled c-myc promoter
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Id2 leaves the chromatin of the E2F4–p130-controlled c-myc promoter

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Id2 leaves the chromatin of the E2F4–p130-controlled c-myc promoter

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dc.contributor.author Rodriguez Filgueira, Jose Luis
dc.contributor.author Sandoval del Amor, Juan
dc.contributor.author Serviddio, Gaetano
dc.contributor.author Sastre Belloch, Juan
dc.contributor.author Morante Hernández, María
dc.contributor.author Perrelli, Maria Giulia
dc.contributor.author Martínez Chantar, María
dc.contributor.author Viña Ribes, José
dc.contributor.author Viña Ribes, Juan
dc.contributor.author Mato, José M.
dc.contributor.author Ávila, Matías
dc.contributor.author Franco Vera, Luis
dc.contributor.author López Rodas, Gerardo
dc.contributor.author Torres Asensi, Luis
dc.date.accessioned 2010-06-29T11:01:52Z
dc.date.available 2010-06-29T11:01:52Z
dc.date.issued 2006
dc.identifier.uri http://hdl.handle.net/10550/14010
dc.description.abstract The Id (inhibitor of DNA binding or inhibitor of differentiation) helix–loop–helix proteins are involved in the regulation of cell growth, differentiation and cancer. The fact that the molecular mechanisms of liver regeneration are not completely understood prompted us to study the fate of Id2 in proliferating liver. Id2 increases in liver regeneration after partial hepatectomy, following the early induction of its gene. Co-immunoprecipitation shows that Id2 forms a complex with E2F4, p130 and mSin3A in quiescent liver and all these components are present at the c-myc promoter as shown using ChIP (chromatin immunoprecipitation). Activation of c-myc during hepatocyte priming (G0–G1 transition) correlates with the dissociation of Id2 and HDAC (histone deacetylase), albeit p130 remains bound at least until 6 h.Moreover, as the G0–G1 transition progresses, Id2 and HDAC again bind the c-myc promoter concomitantly with the repression of this gene. The time course of c-myc binding to the Id2 promoter, as determined by ChIP assays is compatible with a role of the oncoprotein as a transcriptional inducer of Id2 in liver regeneration. Immunohistochemical analysis shows that Id2 also increases in proliferating hepatocytes after bile duct ligation. In this case, the pattern of Id2 presence in the c-myc promoter parallels that found in regenerating liver. Our results may suggest a control role for Id2 in hepatocyte priming, through a p130 dissociation-independent regulation of c-myc. en
dc.language.iso en en
dc.relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559451/pdf/bj3980431.pdf en
dc.source Rodriguez, J.L.; Sandoval, J.; Serviddio, G.; Sastre, J.; Morante, M.; Perrelli, M.G.; Martinez-Chantar, M.L.; Viña, J.; Viña, J.R.; Mato, J.M.; Avila, M.A.; Franco, L.; López-Rodas, G.; and Torres, L. Id2 leaves the chromatin of the E2F4–p130-controlled c-myc promoter during hepatocyte priming for liver regeneration. En Biochem. J. (2006) 398, 431–437 en
dc.subject Cell cycle; C-myc; E2F; Histone deacetylase; Id2; Liver en
dc.title Id2 leaves the chromatin of the E2F4–p130-controlled c-myc promoter en
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Bioquímica en
dc.identifier.doi 10.1042/BJ20060380 en
dc.description.private jorofil@uv.es; sandela3@uv.es; jsastre@uv.es; moherma@uv.es; jvina@uv.es; vinaj@uv.es; franco@uv.es; lopezr@uv.es; torresl@uv.es en
dc.identifier.idgrec 032841 en

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